艾比湖原核微生物菌群结构演变与环境因子关系分析
A Study on the Relationship between the Prokaryote Community Structure Evolution and Environmental Factors of Ebinur Lake 
投稿时间:2019-07-03  修订日期:2019-11-07
DOI:10.12119/j.yhyj.202004010
中文关键词:  盐湖  艾比湖  原核微生物  多样性  系统发育
英文关键词:Salt lake  Ebinur Lake  Prokaryotic microorganism  Diversity  Phylogeny
基金项目:国家自然科学基金(31860149);新疆自然科学基金(2011211B11)
作者单位E-mail
马雪莉 新疆大学资源与环境科学学院,新疆 乌鲁木齐 830046 493112142@qq.com 
吕杰 新疆大学资源与环境科学学院,新疆 乌鲁木齐 830046
新疆大学绿洲生态教育部重点实验室,新疆 乌鲁木齐 830046 
 
马媛 新疆大学资源与环境科学学院,新疆 乌鲁木齐 830046
新疆大学绿洲生态教育部重点实验室,新疆 乌鲁木齐 830046 
 
吕光辉 新疆大学资源与环境科学学院,新疆 乌鲁木齐 830046
新疆大学绿洲生态教育部重点实验室,新疆 乌鲁木齐 830046 
 
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中文摘要:
      用非培养法获得新疆维吾尔自治区艾比湖湖底沉积物原核微生物菌群组成,并与已有盐湖原核微生物菌群数据进行比对,分析湖泊由淡水湖向盐湖演替过程中原核微生物群落结构变化规律。实验获得艾比湖原核微生物16S rRNA基因序列,并从NCBI数据库下载赛里木湖、柴窝堡湖和顿巴斯他乌盐湖3个湖泊的非培养原核微生物16S rRNA基因序列数据。用不同盐湖细菌和古菌16S rRNA序列信息构建系统发育树并与其理化指标进行典型性相关分析。同源比对及聚类结果显示,艾比湖湖底沉积物中细菌包括4个门,拟杆菌门(Bacteroidetes)占克隆文库的64%,变形菌门(Proteobacteria)占9.4%,厚壁菌门(Firmicutes)占3.4%,放线菌门(Actinobacteria)占2.6%,此外含有未分类类群20.6%。古菌含有两个门,广古菌门(Euryarchaeota,98.3%)和盐纳古菌门(Nanohaloarchaeota,1.7%)。不同盐湖系统发育树结果显示,随盐度增加,盐湖细菌从变形菌门向拟杆菌门演替;古菌从奇古菌门和泉古菌门向广古菌门和盐纳古菌门演替。RDA结果显示,Na+、Cl-、SO42-和矿化度对盐湖原核微生物多样性结构起到决定性的作用,K+、Mg2+和Ca2+对艾比湖菌群结构影响作用最为显著。原核微生物群落会随着湖水盐浓度的增加和盐湖化学成分的不同而发生演替。
英文摘要:
      In this study,the uncultured prokaryote diversity of Ebinur lake sediment was identified and compared with other salt lakes to survey the change regulation of the prokaryote community structure during the evolution of salt lakes from freshwater lake to salt lake in Xinjiang Uygur Autonomous Region.The uncultured prokaryote 16S rRNA gene sequences of Ebinur lake were obtained by experiments and the uncultured prokaryote 16S rRNA gene sequences of Sayram Lake,Chai Wo Pu Lake and Dunbasitawu Lake were downloaded from NCBI database.The phylogenetic trees were constructed with these bacterial and archaeal 16S rRNA sequences and the canonical correlation analysis were analyzed with their physical and chemical indexes.The results show that the bacterial clone library could be divided into four phylum,Bacteroidetes,Firmicutes,Actinobacteria and Proteobacteria in Ebinur lake.The phylum Bacteroidetes accounted for 64% of the library,Proteobacteria accounted for 9.4%,Firmicutes accounted for 3.4%,Actinobacteria only accounted for 2.6%,and unclassified bacteria accounted for 20.6% of the library.The archaeal library could be divided into two phylum,Euryarchaeota and Nanohaloarchaeota.The phylum Euryarchaeota accounted for 98.3% of the library,and Nanohaloarchaeota only accounted for 1.7%.The phylogenetic tree results show that the bacterial community evolved from Proteobacteria to Bacteroidetes and the archaeal community evolved from Nanohaloarchaeota and Crenarchaeota to Euryarchaeota and Nanohaloarchaeota with the increase of salinity.The canonical correlation analysis results show that Na+,Cl-,SO2- 4and salt concentrations played a decisive role on the prokaryote diversity during salt lake evolution;K+,Mg2+ and Ca2+ concentration had the significant effect on the Prokaryote community structure in Ebinur lake.The analysis results imply that the prokaryote community structure of salt lake could developed with the increase of salt concentration and the different chemical compositions.
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